Zogwirizana immunosorbent zofufuza: ntchito ndi mbali ya

Zogwirizana immunosorbent zofufuza - phunziro choyezera, ozikidwa pa chizindikiritso cha maofesi "Antigen-oteteza" ndi zolemba wapadera enzymatic kuti kuzindikira, ndi kusintha mtundu wa gawo lapansi ndi. Cholinga cha kusanthula izi - motsimikiza pamaso pa mankhwala osiyanasiyana zochita enzymatic mu zitsanzo mayeso.

Pakuti kudziwika antigens ndi chitetezo sakanikira enzyme immunoassay ntchito, popeza ndondomeko yake n'zosavuta nadzawasankhula zigawo zotani ndi kuchedwa ena a iwo mu gawo olimba.

Dziwani Ubwino wa phunziro zasayansi:

• mkulu tilinazo, amene amatha kudziwa ngakhale ochepa mamolekyulu mwa mankhwala;

• yokwaniritsira kusanthula izi akhoza kugwiritsa ntchito mlingo wa chuma mayeso;

• kuthekera nthawi yaitali kusunga zigawo zikuluzikulu zonse zofunika kwa kusanthula;

• Easy kuchita;

• kupezeka zotsatira malangizo ndi zithunzi;

• immunosorbent zofufuza ndi makina iliyonse siteji zotani;

• wotsika zida ndalama zimene zimagwiritsidwa ntchito pa matenda.

ubwino ntchito kwambiri IFA m'madera osiyanasiyana mankhwala, kuphatikizapo kwa matenda a mavairasi.

Ndondomeko ambiri chifukwa immobilizing chitetezo ndi antigens ndi kumamatira anachita, pamene mamolekyulu osiyana amagwira kwa gawo olimba (chifukwa ankachita amaayoni kapena hydrophobic kapena mwa mapangidwe kumangidwa wa hydrogen).

Ine ndiyenera kunena kuti immunoassay enzyme ntchito zitsanzo ziwiri:

• kudziwa antigens tizilombo toyambitsa matenda matenda osiyanasiyana;

• kudziwa kukhalapo kwa chitetezo ku Antigen tizilombo pathological (tisaiwale kuti Elisa akhoza kudziwa pafupifupi nthenda iliyonse maliseche).

Special polystyrene mbale anamugwiritsa ntchito kuti agwire serodiagnosis izi. Zikuyenera 96 mabowo pa mbali ya Antigen adsorbed. Pa kulowa mu piritsi assayed chitetezo selo Antigen amagwira yoyenera. Kuwonjezera wotsatira wa oteteza kuwanena kuti zitsime za immunoglobulins anthu chimafikira anachita yeniyeni. Otsatirawa chithandizo chromogen seramu (utoto) zimasiyanasiyana ochekenera gawo lapansi (mu kukhalapo kwa maofesi lolingana "Antigen-oteteza"). Dziwani kuti mtundu mwamphamvu ndi mwachindunji njila kwa kuchuluka kwa chitetezo.

Elisa Analysis atsiliza kuyeza kachulukidwe kuwala kwa madzi mu zitsime. Amagwiritsa ntchito zipangizo zapadera zosiyanasiyana stereotyped kuti kuthandiza kuwerengera oteteza ndende. Tiyenera kukumbukira kuti mchitidwe uliwonse mayeso kuchita kusanthula izi ali zake zizindikiro muyezo (ichi zambiri zotchulidwa mu zotsatira, amene anaperekedwa).

Ntchito Elisa oterewa chindoko, chiwindi tizilombo ndi HIV, komanso opezeka thupi cytomegalovirus, mauka, nsungu (osiyanasiyana). kusanthula Izi zimathandizanso kudziwa mlingo wa mahomoni kudziwa zolembera khansa.

Kodi kuti anayesedwa Moscow? Masiku ano, pali zambiri Laboratories apadera, amene kupereka osiyanasiyana mayesero serological. Njira chonde dokotala wa chipatala m'deralo (pawekha kapena katswiri, inu dveryaete). Ndi zotsatira nawonso ayenera kupita kwa dokotala. Ndipo tikamamva ndemanga yake pa chithandizo chilichonse.