Ploskireva sing'anga (Bacto-Hagara F): malongosoledwe. Medical tizilombo tosaoneka ndi maso

Ploskireva sing'anga (hereinafter amatchedwa akadali Bacto Hagara F) ndi sing'anga michere kuti kulima tizilombo ena, makamaka Shigella ndi nsomba. Monga gwero zipangizo ntchito yake kachilombo: mkodzo, ya ndulu, ndowe.

nkhani

Nikolay Ivanovich Ploskirev - Soviet wasayansi ndi dokotala ulemu. Iye anabadwa mu 1869 ndipo anamwalira mu 1948, popeza ntchito kwambiri pa moyo wake kwawo - Tomsk.

Mu 1888, iye, wasayansi m'tsogolo, popanda kumaliza sitandade sikisi a sukulu, usilikali. iye anali akutha kumaliza maphunziro yekha mu 1892, ndiye mayeso a yopemphera kwa Imperial Tomsk University, iye anaphunzira ku mphamvu ya Medicine. Mu chaka cha 1898 iye analandira udindo wa dokotala.

Ploskirev nawo Chirasha Japanese nkhondo, kenako ntchito ku chipatala Tomsk. Mu 1910 anaikidwa kukhala malo mutu wa Tomsk dermatological ndi venereal kuchipatala, ndipo kwa zaka makumi atatu wakhala mutu wake mpaka kalekale. Wasayansi chomwecho anali mmodzi wa omwe adayambitsa opatsirana pogonana chipatala mu Tomsk.

Nikolay Ivanovich Ploskirev analemba mndandanda wa ntchito zoyenera polimbana matenda venereal.

kapangidwe

Lachitatu Ploskireva - mfundo kulima mabakiteriya matumbo, choncho ayenera muli mitundu yambiri ya zakudya. Izo zinabala mu mawonekedwe youma.

Ndithu chiwerengero chachikulu cha kulemera kwake konse kumakhala ndi pancreatic hydrolyzate sprat (10.4 g / l). Pang'ono zochepa nkhani hydrocitrate dibasic ndi sodium (8.5 g / l). Komanso pali youma michere msuzi ndi mkaka shuga (8.62 ndi 7.3 g / l).

Mutu wachiwiri Ploskireva sing'anga - J. Bacto-Hagara mu zikuchokera ndi kupezeka ndi kuchuluka kwa Hagara, 6,94 g / L. Seriovatistokislogo anhydrous ndi sodium zili wofanana 5.1 g / L. Pali pamaso pa anhydrous disodium mankwala - 2.1 g / L, sodium salt asidi cholic za 3,46 g / L ndi koloko phulusa - 2,4 ga

Pasanathe galamu ndi omwe ali m'bukuli zizindikiro - ndale chofiira (0.05) ndi waluso zobiriwira (0.0002). Ayodini zili - 0,13.

ntchito

Kuphunzira njira amuzolengedwa mabakiteriya kumathandiza kwambiri pa matenda a matenda amene amayambitsa zinthu anaerobic. Species m'banja - mazana. Iwo ali pafupifupi ofanana mu kafukufuku wakapangidwe kazachilengedwe ndi katundu chikhalidwe, ndi njira yodalirika kusiyanitsa iwo Chrixitu - ndi kuphunzira sayansi.

phunziro ili likuyamba ndi inoculation zakuthupi pathological mu sing'anga chikhalidwe. Iye pathological masiyanidwe kuti mabakiteriya coliform ndi gawo la izo, kuwonjezera Hagara mbalambanda, zikuphatikizapo kuunika ndi lactose. Processing luso lactose - chizindikiro zofunika zosiyanasiyana za enterobacteria. Ngati azindikira kuti pH akugwiritsa kwa acidity ndi chizindikiro adamulowetsa, amene akutembenukira njuchi.

mayiko ena angathe kugwiritsa ntchito zipangizo zina michere, koma limagwirira zochita limafanana ndi atatu ambiri mu Russia - chilengedwe Endo ndi Levine Ploskireva Lachitatu.

Kufotokozera za njira

Lina Ploskireva analogi sing'anga ndi otchedwa Macconkey Hagara. Mu yomalizidwa mawonekedwe njira mandala ali kuwala pinki-chikasu. M'midzi akhoza pokonza lactose, mwa Ploskireva kupereka wofiira (kiranberi) mtundu. Ngati mabakiteriya sangathe pokonza, kapena njuchi ndi colorless kapena ali ndi mtundu ofooka.

Popeza kuti zikuchokera sing'anga zikuphatikizapo zinthu Ploskireva zoletsa (waluntha wobiriwira, salt ya ndulu, ayodini), ndi pafupifupi kwathunthu kumabwezera mmbuyo kukula kwa zomera galamu HIV, monga woyamba tsiku eshehiry kwambiri retards kukula, komanso ena mwachizolowezi concomitant microflora.

mwendo yachiwiri

Komanso chidwi ndi masankhidwe a njuchi, ndipo seeded pa pulayimale yosungirako sing'anga ndi zosiyanasiyana. Environment limene pali seeding ayenera kukhala ndi magawo angapo. chikhalidwe bakiteriya ayenera kudziwa ntchito enzymatic ndi ulemu kwa iwo, kwa sing'anga yomweyo anaikidwa machubu kuti pali malo awiri:

• imodzi imene slanting Hagara;
• ndi mzati.

The chidwi ofufuza ku njuchi asezare bevelled gawo wandiweyani sitiroko ndi mzati - pokha. Pa nthawi imeneyi, a sing'anga ntchito Russell, Kligler kapena Olkenitskogo. Monga masiyanidwe matenda ntchito ndi Ploskireva Lachitatu.

Microbiology ndi tizilombo zamoyo

Hagara Ploskireva ndi chinthu chofunika kwambiri kupeza matenda oyamba ndi Enterobacteriaceae. Mwachitsanzo, izo wakula ndi kusiyanitsidwa chambiri tizilombo amene amayambitsa bacillary kamwazi. Izi zamoyo anaerobic a mtundu Schigella.

Ngati aliyense amene ali mbali ya banja la tizilombo m'mimba, shigella ndi mawonekedwe a ndodo, kukula kwa micrometers awiri. Sindiwo makapisozi ndi spores ndi michirayo imakhala yosiyanasiyana - timatha iwo kulekanitsidwa kwa nsomba, amene nawonso, ndi mafoni. Iwo amakula bwino pa TV zophweka kwambiri, pa kutentha pang'ono pamwamba yozungulira (35-37 ° C) ndi 7,4 pH. Ndi chikhalidwe cha kukula, madera musati amasiyana nsomba.

Monga kudafotokozedwa pamwamba, tizilombo akhoza kukhala zayamba morphological kusiyana Komabe, zimasiyana kwambiri pa ndondomeko amuzolengedwa wa moyo. Choncho, kupatula Shigella Nyukestl, popanda mapangidwe mafuta asidi zidzachitika nayonso mphamvu ya chakudya. Kupatulapo Shigella sonnei, iwo sangakhoze kupesa lactose, koma m'mimba shuga. Monga mbali awo kiyi, mukhoza n'kuwatengera zimene angathe kuchepetsa niraty kuti nitrite. madera awo m'mimba ndi urea, komanso mu sing'anga chikhalidwe palibe liquefaction wa gelatin.

Collection ndi yokonza mbewu

Kudziwa nthumwi za kamwazi, zifunika mayesero microbiological wodzala mapangidwe, mwachitsanzo ndowe mtima. Pa chiphaso cha chuma, muyenera mwamsanga kuchita kufesa. Ngati zimenezi sizingatheke, gwero ayenera kuyikidwa mu usawonongeke - mankwala gawo lotetezedwa osakaniza kapena glycerol. Pa 4 ° C, iwo akhoza kusungidwa zoposa tsiku. Sungani zinthu ziyenera kuperekedwa kudzera mwa thumbo galasi chubu amene anaikapo mu rectum.

Maphunziro zakuthupi anasankha zidutswa purulent ntchofu kutsukidwa machubu awiri kapena atatu isotonic sodium kolorayidi njira.

Kugwiritsa tizilombo toyambitsa matenda chilengedwe Ploskireva anapanga galasi spatula. Muyenera opaka pa gawo laling'ono la Hagara kwake, ndiye Vulani spatula pa chilengedwe, ndi chuma yotsalira ndi kuzitikita mu pamwamba zija. Ngati seeding chikachitika mu makapu angapo, ndiye kuti aliyense wa iwo ayenera kudzala mtanda watsopano.

Ngati muco-purulent zidutswa pali kutenga pa kusanthula odzipatulira, sizitanthauza kuti palibe kukhalapo kwa tizilombo toyambitsa matenda. Zikatero m'pofunika emulsify ndowe mu 10 ml ya sodium kolorayidi (ndende 0,85%) ndi ndiye kubzala madontho limodzi kapena awiri pa sing'anga Ploskireva. mpando Non-emulsified zingafesedwe mu msuzi selenitovy. Iye ntchito, ngati, mmalo mwa ndowe ndi masanzi kapena washings.

matenda microbiological

Mu gawo loyamba la maphunziro microbiological ikuchitika yofesa tizilombo toyambitsa matenda mu makapu awiri, ndiye kuti penyani momwe kukula Shigella. Lachitatu Ploskireva kutulutsa mbewu, ndipo chachiwiri - pa malo kapena Endo Levin.

Chifukwa chakuti panali tizilombo ta shigella ndi kugonjetsedwa ndi mankhwala kwa sing'anga anali wodzilemekeza ndi chloramphenicol. Ndiye kumachitika mwa masiku makulitsidwe mu chofungatira pa 37 ° C.

Pa tsiku lachiwiri kufufuza madera wamkulu. Amene sali pa masiyanidwe matenda sing'anga wamkulu colorless, m'pofunika kulimira Russell sing'anga kapena lalifupi "motley mzere." kafukufuku ena imachitika ndi aligorivimu mbewu yaikulu pa iwo. Chambiri Shigella Lachitatu Ploskireva kukula mu mawonekedwe a mandala, colorless ndi zazing'ono mizati. Iwo ali a mitundu iwiri:

• flattened m'mbali losongoka;
• anamaliza, otukukira kunja, ndi mmene yonyowa kuwala.

madera atatu kapena anayi ayenera mikroskopirovat ndipo anafufuza kwa sayenda. Ngati yotsirizira sangosonyeza, iwo adasamutsidwa Lachitatu Olkenitskogo kupeza chikhalidwe choyera. Popeza madera khalidwe Shigella kapena ngati palibe kukula anati konse, m'pofunika Endo Hagara kapena Ploskireva zokolola seeding a selenitovogo msuzi. Ngati madera lililonse alipo chiwerengero chokwanira, kuika Akuti agglutination. A chisakanizo cha Sera sonnei ndi Flexner, anachita limapezeka pa galasi.

mudziwe zambiri

Pa tsiku lachitatu pali kusintha amene anachitika mu madera pa sing'anga Russell. Ngati pali chikhalidwe si amanyoza lactose ndi shuga chimafufumitsa kubala asidi, izo ndi wosiyana ndi kusanthula gwiritsirani wamamamayikirosikopu, komanso kugwira kufesa mu "mzere motley," koma nthawi iyi kukodzedwa. Kungoika agglutination anachita, kuti chizindikiritso serological.

Pa tsiku lomaliza, Ndizachidziwikire pa maziko a kusintha mu "chiwerengero motley" (ngati pali nayonso mphamvu ya chakudya), komanso mwachidule agglutination anachita.

Yosungirako ndi kukonzekera

Kukonzekera Hagara Ploskireva kumachitika motere:

1. 55 ga mankhwala youma akawinduka mu lita imodzi ya madzi otchezedwa.
2. Owiritsa kwa mphindi ziwiri kapena zitatu mpaka Hagara sanayambebe kwathunthu kusungunuka.
3. Thirani mu petri mbale (sterility optional) wosanjikiza wa 5-6 mm.
4. Mbale ali zosatseka kwa theka la ora firiji (18-25 madigiri). Patapita nthawi iyi sing'anga ndi mokwanira asamaumire ndi youma.

Lachitatu Ploskireva photosensitive ndi hygroscopic. ufa ziyenera kusungidwa mu phukusi womata, chinyezi wachibale mu chipinda sayenera upambana 60%. Kutentha akadakwanitsira zinthu kwa kusungiramo 2 ° C kwa 25 ° C. Yosungirako ayenera kutsatira malamulo amenewa, mwinamwake zotheka anapezazo zolakwika.