Maselo Diagnostics: zenizeni nthawi PCR

Panopa diagnostics maselo limagwirira cholinga polymerase unyolo anachita (PCR), amene anali kutsogolera kwa kukula kwa chiwerengero chachikulu cha njira ya matenda a matenda, zochokera kuona zinthu chibadwa. Yokwaniritsira zenizeni nthawi PCR ntchito jini mpukutu (makamaka chidulo mkati) wa wothandizila causative, omwe sapezeka anthu ena.

Mfundo PCR ndi chulutsa wina tizilomboto DNA mpukutu mamiliyoni ambiri mwa DNA polymerase mu gulu lapadera, amatchedwa cycler ikukhudzana amene amathandiza kubwereza ozungulira ulamuliro wa kutentha pulogalamu anapatsidwa.

Diagnostics PCR amalola kubwereza mndandanda wa amplifications, kukhalabe ntchito enzyme. Pamene amveke chikuchitika bwino, mu dongosolo lina chiwerengero cha magazini a chidutswa makamaka a DNA wa awiriawiri tizilomboto, pambuyo m'zinthu makumi atatu - zana ndi khumi. N'chifukwa chake PCR ntchito matenda kudziwa yaing'ono tizilombo toyambitsa matenda mokwanira, chizindikiritso cha zomwe n'zosatheka popanda izo.

Tikumbukenso kuti RNA sangakhale Chinsinsi PCR mu nthawi yeniyeni. Choncho, pofuna kudziwa RNA tizilombo pamaso amveke ndi kuti DNA chilamulira chikhalidwe ndi mpukutu lolingana za izo. The polymerase unyolo anachita amalola kubala DNA ndi RNA muyeso tizilomboto. Mfundo Choncho analandira ntchito yowunikira mphamvu ya mankhwala ntchito, ndi kufufuza matenda madokotala ananena zawo.

Komanso, zenizeni nthawi PCR sikutanthauza zochita zina ndi kulemekeza decryption wa chandamale DNA kapena amplicons, zimene zikulepheretsa kuti matenda lolondola ndi kuyambitsa zotsatira zabodza. njira Izi zimathandiza kuti ntchito electrophoresis sitepe, imbaenda ku mankhwala ngozi kuchepetsa wa polymerase unyolo anachita. Komanso, chifukwa cha kuchepa kwa chiwerengero cha mwai ndi wothandizila pa, amachepetsa nthawi ya kafukufuku, izo zimatithandiza ndondomeko, amachepetsa imabwera zolakwika. PCR komanso zimathandiza kuti mwachindunji nambala yoyamba ya mabuku a DNA wa tizilomboto pa m'chitsimikizo matenda kuthandiza kuzindikira nyengo exacerbations ndi kukhwimitsa yake kuchiza wodwala.

Masiku ano, pali lalikulu nambala ya amveke njira fixation mu weniweni nthawi. The zambiri ntchito njira zosiyanasiyana kutsatira amplicon chilamulira chikhalidwe ndi probes fluorogenic, ndi cheza mwamphamvu amene imasiyana malingana ndi kudzikundikira amplicon analembedwa pompopompo mwa chipangizo wapadera wotchedwa thermocycler ndi gawo kuwala. Imakhala mkulu mlingo wa tilinazo kuwunika. Pa yemweyo PCR mankhwala angathe kuonedwa, pamodzi ndi chiwiya-mankhwala ndi, pa nthawi imene ntchito electrophoresis mu zina si anaona.

Ndi PCR ntchito zabwino ndi zoipa ulamuliro zitsanzo mu nthawi yeniyeni. Choncho, zabwino ndi zoipa zitsanzo kuphunzira kusanthula ngati osiyana zitsanzo, ulamuliro chitsanzo momwe umayamba aliyense ngati chitsanzo napereka zonse kusanthula njira.

Masiku ano, pali ambiri zipangizo zogwiritsa kunja kwa polymerase unyolo anachita. Zikuphatikizapo Bio-Rad, Akutsatira Biosystems, Cepheid ndi Roche. Aliyense ali ndi ubwino ndi kuipa

Tikumbukenso kuti PCR - njira matenda ntchito zikuluzikulu za sayansi, kufufuza ndi malo matenda padziko lonse, chifukwa amapereka mwayi kugwira kutanthauzira msanga zotsatira zake.