TLC yosavuta njira kusanthula zovuta

TLC ndi mtsogoleri nkhani ya kusanthula kachulukidwe ndi theka-kachulukidwe wa mankhwala zovuta, zachilengedwe, Biomedical, sayansi, mankhwala ndi zinthu zina zambiri. Woonda wosanjikiza koromatogarafe ndi njira kwambiri lomwenso a kusanthula Unyinji wa pafupifupi magulu onse a zinthu.

Njira imeneyi ya kupatukana ndi kusanthula machitidwe Mipikisano chigawo ankagwiritsa ntchito Laboratories wa kulunjika osiyana kwambiri ndi malangizo: ukhondo-AIDS, Chowona Zanyama, agrochemical kafukufuku, azamalamulo, ndi za malo chitetezo zomera, Center kwa Standardization ndi Bureau ya azamalamulo Medicine.

Ubwino waukulu wa woonda wosanjikiza koromatogarafe, zochokera zoyenda eluent (analyte zosungunulira) mu sorbent wosanjikiza mwa wotchedwa mphamvu capillary ndi kuphweka ndi easiness za ndondomeko chromatographic, mlingo yapamwamba zedi yolinganizika wa kusanthula ndi mtengo wotsika wa zida zina zofunika.

TLC, ndi digiri yapamwamba ya osiyanasiyana (m'munsi kudziwika pakhomo) ndi selectivity, zimathandiza kudziwa 10-20 ug zinthu mpaka 7%, omwe ndi mkulu. Woonda wosanjikiza koromatogarafe akhoza kukhala kumamatira ndi kugawa mitundu. Ambiri ntchito njira yoyamba.

Akamanena za njira lili mu TLC zosungunulira mankhwala chimachititsanso mu sorbent bedi ndi kugawa okhudza malo a zigawo zosiyanasiyana malinga ndi makhalidwe awo ndi katundu. chakuti mamolekyulu wa zosiyanasiyana ndi kapangidwe ka adsorbed ndi (adaikanso) padziko mbale olimba chromatographic mosiyana. Choncho, zovuta machitidwe Mipikisano chigawo pang'onopang'ono anapatukana mu zigawo zikuluzikulu.

Kupita imathamanga osiyana pamodzi sorbent bedi, munthu kale analekanitsidwa, zigawo kupanga chromatogram. Aliyense mtundu malo limafanana ndi mankhwala enieni. Colorless chinthu ndi kuwala ukutitsogolera limene kuchuluka kwa mankhwala anayamba fluoresce, kapena pansi wapadera mankhwala ochekenera reagent.

Mwa masiku ano njira mawunikidwe GLC chionekera. Njira imeneyi amakhala ndi kulekanitsidwa ndi kusanthula ntchito, monga sorbing wothandizila wapadera nonvolatile madzi, mmene ufa zinthu ceramic ndi wasambitsa. Ndipo ngati sing'anga galimoto ntchito pano osiyana mpweya inert. Njira imeneyi ndi yabwino kwambiri kuphunzira zosakaniza zovuta kuti muli mazanamazana a zigawo zosiyana.

Wina ano njira kufufuza zikuchokera zinthu zovuta ndi madzi koromatogarafe. Mbali yaikulu ya njira imeneyi imakhala mu kulekana kwa zigawo zikuluzikulu pogwiritsa ntchito kusiyana yogawa awo pakati pa makina (eluent) ndipo anakakonza (adsorbent) mbali. Njira imeneyi nthawi zambiri ntchito mu kusanthula mankhwala osiyanasiyana organic (zina chakudya, mankhwala osiyanasiyana poizoni, etc.). Komanso, monga njira chromatographic ntchito kuphunzira ulamuliro amasokoneza mankhwala, mtima wa mankhwala mu madzi zamoyo ndi zina kuwunika kwa amino acid.