Vitrification ya mazira pa onyamula munthu

M'nkhani ino tikambirana mfundo monga vitrification ya mazira. Dr Kuwayama Masashige kupangidwa njira imeneyi kriotopah ngakhale mu chaka chaka. The mwana woyamba kubadwa mwa mazira vitrification mu 2003. oocyte mlingo kupulumuka anali chinawonjezeka ndi 98 peresenti.

Theka la akazi amene alandira lidzawagwiritsenso, mazira kukhalabe. Pakuti iwo unachitikira cryopreservation kuti amalola odwala ndalama. Ndipotu unfreeze ndi kusamutsa mazira ndi mosavuta kuposa kuchita mu ndondomeko m'galasi umuna kachiwiri. Ndi mtundu wa inshuwalansi mu nkhani ngati mkazi sakhala ndi mimba. Cryopreservation ali ndi mwayi osiyana - kuteteza imfa ya mazira yotheka amene anatsalira protocol lapansi.

ontogeny

Pa chitukuko zotengera malingaliridwe a chamoyo, kapena ontogeny, izo unalembedwa pa nthawi ya umuna ndipo umatha ndi imfa yake. Kuyendetsa kumeneku ndi mosalekeza mu nthawi ndipo ali ndi khalidwe okhazikika. Ndipo sitingathe ake suspend kapena m'mbuyo chitukuko. Koma mwachibadwa pali kuchotserapo. Awa ndi zomera, invertebrates ndipo ngakhale ena inakhala pulayimale kuti pa kutentha otsika, musati kuonetsa katundu khalidwe la zamoyo.

hibernation ndi chiyani?

Vitrification ya mazira zafotokozedwa pansipa. Munthu nthawi ya bata amatchedwa hibernation. Mwachitsanzo, nyama zambiri apulumuke kutentha Siberia kufika madigiri -90, ndi madzi m'thupi pafupifupi onse. Mu kuphunzira imeneyi ontogenesis m'chilengedwe pali funso za ntchito zotheka wa otsika kutentha kwa kusokoneza tsankho ndi kusintha kwa ntchito kwa zolengedwa apamwamba peresenti, kuphatikizapo anthu.

cryopreservation

Cryopreservation - ndi njira zothandiza kuyimitsidwa njira kwachilengedwenso maselo malinga ndi kutentha otsika. Izi zimasunga ntchito moyo maselo pa magetsi. Ndi kutchuka, njira imeneyi ndi otsika Ndisanaumbidwe vitrification. 1 kriotop (zaikidwa krionositel) lili ku mazira 1 mpaka 3.

Mwachitsanzo, pamene kuchita ndondomeko monga lidzawagwiritsenso, zotsatira zabwino imatengedwa kuti akusuntha mu uterine M'mimbamo si mazira oposa awiri. Otsala mazira khalidwe zikhoza cryopreserved ntchito m'tsogolo. Iwo akhoza kufunsira kukonzanso lidzawagwiritsenso patapita kanthawi, ngati ndondomeko adzakusonyezani chifukwa negative. Ndi zolinga zimenezi anagwira vitrification mazira pa onyamula payekha.

Nthawi zina, amaundana mazira onse. Kwa amayi amene ali yamchiberekero hyperstimulation syndrome pa superovulation kupatsidwa ulemu imagwiridwa zambiri. Ndani analimbikitsa yozizira koopsa? Odwala matenda a khansa, makamaka pamaso pa ndondomeko mankhwala amphamvu kapena radiotherapy. deta ndiye anasamutsa mazira mu uterine patsekeke. Amaundana amasonyeza aliyense amene mwayi wa mimba pambuyo lidzawagwiritsenso pazifukwa zina adatchithisira. Izi zikhoza kukhala endometrial tizilombo ting'onoting'ono, osakwanira endometrial makulidwe nthawi inakonzedwa kutengerapo, osokonekera magazi.

masiteji yozizira koopsa

mazira anali atapanga pamisinkhu yosiyanasiyana:

• ubwamuna dzira (zygote);
• onongani mazira siteji;
• blastocysts.

Pa nthawi, pali njira ziwiri za mazira ndi kuzizira.

wosakwiya yozizira koopsa

Vitrification ya mazira ikuchitika pa yozizira koopsa pang'onopang'ono. njira koteroko akufuna mu 70s ndi imodzi mwa njira zoyambirira chakale cha mazira kuzizira. Bukuli lili kuzirala pang'onopang'ono pa mlingo zonse. Kamodzi mazira ndi kusungidwa asafe.

Koma tisaiwale kuti pa wosakwiya yozizira koopsa mu njira cryoprotective timibulu tosaoneka ayezi anapanga amene kusokoneza maselo a kathupi lapansi. Zingayambitse tsankho kapena okwana kuwonongedwa kwa zinthu zamoyo pa magetsi. chiwerengero cha mazira bwinobwino anasamutsa pa yozizira koopsa wodekha ndi thawing, pafupifupi 70 peresenti.

vitrification

Pambuyo 2010, anayamba kugwiritsa ntchito njira yatsopano ndi aluso cryopreservation - vitrification. Poyerekeza ndi njira yapita, ino ndi njira wapamwamba-mwamphamvu amaundana zinthu zamoyo. nthawi zambiri kumachitika mazira pambuyo vitrification PGD (matenda majini).

Pamene ntchito ili ndondomeko cryoprotective njira, kumene mazira anayikidwa si kupanga timibulu ayezi pa kuzizira. Choncho, yafupika azima Mwina wosabadwayo. Kutsogoza njira si njira ya yozizira koopsa, komanso mlingo apulumuke mazira pambuyo thawing. Malinga ndi kafukufuku, chiwerengero cha kupulumuka mazira pambuyo ndondomeko vitrification si zosakwana peresenti 95.

Kodi chimachitika pambuyo thawing?

Pambuyo thawing mazira musati amasiyana mazira wamba. Iwo komanso mizu ndi kukula. Ndi Kutentha mazira onse pansi pa kuswa anathandiza. Kuchititsa opaleshoni pamwamba wosanjikiza Ndisanaumbidwe adzagaŵikana laser mtengo pa ngodya anakhumba ndi otetezeka. Izi facilitates Ndisanaumbidwe mwa chipolopolo ndi kumawonjezera kuthekera kupatsidwa zina bwino mu chiberekero.

Yozizira mazira zimathandiza kusunga kwa nthawi yaitali. Zimenezi ndi yosafuna zambiri, chifukwa mtengo wa kuteteza thawing ndi kuphunzitsa Ndisanaumbidwe m'chiberekero ndi zosakwana ndondomeko yachiwiri mu m'galasi umuna.

Vitrification imatengedwa ngati kusintha phased kumene njira ozizira pansi yozizira pansipa kutentha galasi kusintha. Pa nthawi yomweyo patsalanso dongosolo amorphous a galasi ndipo afika khalidwe lofanana ndi zolimba crystalline. Choncho, maselo onse wamoyo, Ndisanaumbidwe lonse adziwonetsa "galasi". Kunyezimira Kapangidwe ka madzi analandira pansi vitrification chifukwa kuzirala ake mofulumira, i.e. mphamvu zosagwiritsika ntchito ya madzi yafupika nthawi yaifupi nthawi kuposa zofunikira dongosolo mphamvu zosagwiritsika ntchito galasi.

Mu mawu osavuta, madzi amaundana ngati mphamvu zosagwiritsika ntchito njira yake mphamvu zosagwiritsika ntchito galasi. Koma kuti bwino vitrified zamoyo, m'pofunika kukwaniritsa mlingo wa kutentha sakuyendetsa ≈ 108 ° C / min., Ndipo n'zosatheka kuchita, chifukwa ntchito cryogenic madzi singakwane kutentha izi, ndipo sangathe ntchito vitrifitsiruemy yothetsera mu buku kakang'ono kuposa voliyumu oocyte. Izi zonse zikusonyeza ndi vitrification ya mazira. Kodi izo, tsopano kapena sichidziwika bwino.

Asayansi anatha kutsimikizira kuwonjezeka Lachitatu kwa amaundana cryoprotectants limakupatsani msanga kuchepetsa liwiro yozizira koopsa. Choncho, pa osalimba a 10% propylene glycol ndi etilengligolya liwiro yafupika kwambiri, pa 40 peresenti ya osalimba a vitrification n'zotheka pa mtengo yozizira 10 ° C / Mph, ndi pa 60% mlingo akutsikira 50 ° C / min. Koma ndi kuwonjezeka kachulukidwe wa cryoprotectants, kugwa Lachitatu, kuwonjezeka zimakhudza awo olakwika pa yozizira koopsa wa biomaterials. Wosakwiya yozizira koopsa amakwiya pakhale madzi chilled mu chamoyo kwachilengedwenso ndi mchitidwe okhudza maselo ambiri. Chotero chikhalidwe kumachitika chifukwa maselo aakulu madzi m'thupi ndi maonekedwe a ayezi extracellular.

Choncho, pa chiphaso cha nyumba vitreous kuswa mankhwala ndi njira thupi la madzi m'thupi. Ngakhale kuti vitrification ya mazira (ndiko kuti, wakhala anafotokoza pamwamba) ndi kovuta kwambiri dongosolo thupi, zipangizo za nyumba imeneyi angapezeke mu moyo wathu tsiku ndi tsiku (galasi, pakachitsulo, ndi zina zotero).

Vitrification ya mazira: Ndemanga

Njira amatenga maganizo chabe positive. vitrification ndondomeko ndi zotheka. Koma kwambiri mbali mu misinkhu yosiyanasiyana mu lidzawagwiritsenso Laboratories. Vitrification - si njira yatsopano ya cryopreservation maselo amoyo. Iye ndi sitepe wotsiriza wa yozizira koopsa pang'onopang'ono. Masiku ano, akazi ambiri amatha kukhala ndi mwana chifukwa cha zinthu sayansi.

anapezazo

Chifukwa ntchito ambiri asayansi vitrification angathe kuchitidwa popanda mtengo mufiriji analinganiza ndi ntchito zida zing'onozing'ono zimene oyang'anira ntchito. Choncho, njira ya m'Chingelezi chosavuta ndi zotsatira bwino. Ngakhale kutukuka kwakukulu mu cryopreservation, kukhazikitsa zachilengedwe moyenera zamoyo pa kutentha otsika lero n'zosatheka.